The endosymbionts of Glossina morsitans and G. palpalis: cultivation experiments and some physiological properties.
نویسنده
چکیده
Pyruvate, malate, and succinate are the main substrates for bacteroid respiration; oxygen uptake can be inhibited by rotenone and antimycin A, but not by cyanide. The symbionts displayed limited growth and survival for over 80 days in a medium with succinate and pyruvate as main substrates, and supplemented with nucleotides. It was not possible to cultivate the endosymbionts of G. morsitans and G. palpalis intracellularly in cell cultures of the tsetse fly or of vertebrates. A high attraction between cells and symbionts was observed in these systems; about 10% of all bacteroids were incorporatedby the cells but they were lysed and digested within 48 h.
منابع مشابه
Two Tsetse fly species, Glossina palpalis gambiensis and Glossina morsitans morsitans, carry genetically distinct populations of the secondary symbiont Sodalis glossinidius.
Genetic diversity among Sodalis glossinidius populations was investigated using amplified fragment length polymorphism markers. Strains collected from Glossina palpalis gambiensis and Glossina morsitans morsitans flies group into separate clusters, being differentially structured. This differential structuring may reflect different host-related selection pressures and may be related to the diff...
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African trypanosomes undergo a complex developmental process in their tsetse fly vector before transmission back to a vertebrate host. Typically, 90% of fly infections fail, most during initial establishment of the parasite in the fly midgut. The specific mechanism(s) underpinning this failure are unknown. We have previously shown that a Glossina-specific, immunoresponsive molecule, tsetse EP p...
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Tsetse flies from the subspecies Glossina morsitans morsitans and Glossina palpalis gambiensis, respectively, transmit Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense. The former causes the acute form of sleeping sickness, and the latter provokes the chronic form. Although several articles have reported G. m. morsitans gene expression following trypanosome infection, no comparab...
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عنوان ژورنال:
- Acta tropica
دوره 36 3 شماره
صفحات -
تاریخ انتشار 1979